10x-pert Workshop - How to: Single-Nuclei RNA-seq

Posted By: shauna-10x, on Nov 30, 2017 at 2:27 PM
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On November 28 we hosted our 6th 10x-pert Workshop: How to: Single-nuclei RNA-seq featuring 10x R&D scientists Sharmila Chatterjee and Paul Ryvkin. Sharmila presented our single-nuclei isolation demonstrated protocol and discussed some of the differences between single-nuclei and single-cell RNA-seq. Paul discussed single-nuclei data analysis and gave a great live demo of Loupe Cell Browser featuring one of our single-nuclei datasets. Following the presentation, Sharmila and Paul answered audience questions during an open Q&A session.

 

Watch the session recording and find links to single-nuclei and single-cell RNA-seq resources below.

 

Recording table of contents:

  • Why choose single-nuclei RNA-seq:  2:50 - 7:05
  • Nuclei sample preparation and best practices: 7:05 - 25:35
  • Overview of single-nuclei RNA-seq data analysis: 25:35 - 37:15
    • Loupe Cell Browser demo with nuclei dataset
  • Open Q&A session: 37:15 - 56:30

 

 

 

Single-cell RNA-seq resources:

Have questions? Ask them in our Single Cell forum.

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