Highly multiplexed transcriptome and protein immune profiling in single cells with CITE-seq
CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by sequencing) is a method in which oligonucleotide-labeled antibodies are used to integrate cellular protein and transcriptome measurements at a single-cell level, with the number of protein markers assayed simultaneously far surpassing what can be measured by cytometry-based methods. Dr. Smibert and his colleagues at the New York genome center originally described this method last year and demonstrated its compatibility with the 10x Single Cell Gene Expression solution.
This Nature Webcast focuses on newly tested applications and recent updates to CITE-seq including:
- Application to human T cell populations using 83 tagged antibodies in a single experiment
- Demonstrated compatibility with the 10x Single Cell Immune Profiling Solution
- Cell hashing, a method using CITE-seq reagents for sample multiplexing