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From tips and tricks to 10x news, our blog is here to help you learn about all things 10x. Let us know what topics you would like to see in the Community Suggestions forum.

The Chromium Single Cell Immune Profiling Solution is a comprehensive approach to simultaneously examine the cellular context of the adaptive immune response and the immune repertoires of hundreds to millions of T and B cells on a cell-by-cell basis.  In this workshop 10x-perts Stephane Boutet and Sarah Taylor discussed how the Single Cell Immune Profiling Solution can be used to characterize the tumor microenvironment.
Linked-Reads and the Supernova Assembler enable low-cost, everyday diploid de novo assemblies. In this workshop 10x-perts David Jaffe and Nikka Keivanfar discussed Supernova assembly performance across a range of species, including human, insect, plant and vertebrates, and highlighted improvements to our high molecular weight DNA sample preparation protocols, including a new protocol for single insects.
Single-cell RNA-seq enables researchers to examine gene expression on a cell-by-cell basis providing insights into the function and regulation of complex biological systems.  In our recent 10x-pert Workshop, R&D scientists discussed single-nuclei RNA-seq, including when to choose single-nuclei RNA-seq, nuclei isolation protocols and best practices and data analysis considerations.
Profiling paired V(D)J transcripts from hundreds to millions of lymphocytes on a cell-by-cell basis, the Chromium™ Single Cell V(D)J system is a complete, scalable tool that has the potential to accelerate progress in immune therapy development.  In our recent 10x-pert Workshop, R&D scientists discussed the V(D)J Solution and performed a live demo of Loupe™ V(D)J Browser for data visualization.
Single-cell RNA-seq enables researchers to examine gene expression on a cell-by-cell basis providing insights into the function and regulation of complex biological systems.  In our recent 10x-pert Workshop, R&D scientists discussed single-cell RNA-seq data analysis tools, including Cell Ranger™ analysis pipelines, and performed a live demo of Loupe™ Cell Browser for data visualization.
Linked-Reads provide long-range information enabling structural variant detection and phasing of genome and exome sequencing data.  In our third 10x-pert Workshop session, our R&D 10x-perts provided an overview of the Loupe™ Genome Browser for structural variant detection and took closer look at visualizing simple structural variants and deriving complex events.
High quality high molecular weight (HMW) DNA is key to getting the most out of Linked-Read data. In a recent 10x-pert Workshop session, our R&D 10x-pert discussed general tips and best practices for isolating HMW DNA and took a closer look at two of our demonstrated protocols for HMW DNA isolation from fresh frozen tissues and DNA size selection.
We recently hosted our first live, online 10x-pert Workshop featuring single-cell sample prep topics.  Two of our R&D scientists gave a presentation detailing general do’s and don’ts followed by a closer look at two of our demonstrated protocols for preparing nuclei for single-cell RNA-seq and removing dead cells.