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From tips and tricks to 10x news, our blog is here to help you learn about all things 10x. Let us know what topics you would like to see in the Community Suggestions forum.

Linked-Reads provide long-range information enabling structural variant detection and phasing of genome and exome sequencing data.  In our third 10x-pert Workshop session, our R&D 10x-perts provided an overview of the Loupe™ Genome Browser for structural variant detection and took closer look at visualizing simple structural variants and deriving complex events.
High quality high molecular weight (HMW) DNA is key to getting the most out of Linked-Read data. In a recent 10x-pert Workshop session, our R&D 10x-pert discussed general tips and best practices for isolating HMW DNA and took a closer look at two of our demonstrated protocols for HMW DNA isolation from fresh frozen tissues and DNA size selection.
Have you ever wondered how to best prepare your samples or how much sequencing you need to achieve the highest quality Linked-Read data? We recently published a Bench Tips article with Biocompare, where we highlight important tips to make the most of our Linked-Read technology for long-range genomic analysis.
The large scale and repetitive nature of conifer genomes make them an important benchmark for assembly technologies. At 31 Gb, the Pinus lambertiana (sugar pine) genome is the largest genome assembled to date. Using the GemCode™ System from 10x Genomics, researchers at UC Davis scaffolded the de novo assembly and produced a new reference assembly for sugar pine with 8-fold improvement in contiguity.
Check out our growing list of resources and learn how to make the most of the human genome with Linked-Reads.
The Chromium™ Genome and Exome Solutions use Linked-Reads to provide long range information from short read sequencers, giving you the power to detect complex SVs.
In a recently published paper, entitled “Assembly of the Complete Sitka Spruce Chloroplast Genome Using 10X Genomics’ GemCode Sequencing Data” researchers used GemCode’s novel Linked-Read data type for de novo assembly of a complete chloroplast genome.
Linked-Reads provide the long range information missing from standard short read sequencing approaches, enabling reconstruction of long range haplotypes, detection of complex structural variants and the ability to perform de novo genome assembly.
  We are excited to introduce the latest evolution in 10x Chromium Software, Supernova 1.1, Long Ranger 2.1 and Loupe 2.1. 
The ability to routinely assemble phased de novo human genomes will help provide a more complete picture of human genetic variation, especially for complex structural variations.