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From tips and tricks to 10x news, our blog is here to help you learn about all things 10x. Let us know what topics you would like to see in the Community Suggestions forum.

Our 3rd North American User Group Meeting of the year took place on Thursday, April 26th, in Chicago, and we were thrilled to see so many members of the 10x community there!
AACR 2018 is officially over! It was a great conference and the last day was no exception. 
AACR 2018 might be more than halfway over, but it didn’t show any signs of slowing down today. It was another jam-packed day full of highlights, starting with a Symposium on cell fate and treatment response
From posters to talks to our Exhibitor Spotlight co-sponsored with Miltentyi Biotec Day 2 at AACR 2018 was all about single cell genomics!
Today was a big day for 10x at AACR 2018! Not only did I get to check out a bunch of great posters and talks featuring 10x technology, but I also got to attend the 10x Genomics Workshop, “Revealing the True Biology of Cancer."
We are thrilled to announce the launch of the Chromium Single Cell CNV Solution. Combing high-throughput single cell profiling with genome-wide copy number variation (CNV) information, our new solution enables researchers to reveal genomic heterogeneity and understand clonal evolution.
The Chromium Single Cell Immune Profiling Solution is a comprehensive approach to simultaneously examine the cellular context of the adaptive immune response and the immune repertoires of hundreds to millions of T and B cells on a cell-by-cell basis.  In this workshop 10x-perts Stephane Boutet and Sarah Taylor discussed how the Single Cell Immune Profiling Solution can be used to characterize the tumor microenvironment.
Please join us on Tuesday, March 20th for the next 10x-pert workshop: Characterization of the tumor microenvironment with the Chromium Single Cell Immune Profiling Solution.
In a new publication in Blood Advances, researchers utilized the Chromium™ Single Cell 3’ Gene Expression Solution to explore immune surveillance in multiple myeloma and illustrate a novel pathway where immunomodulatory drugs may modulate the immune microenvironment.
The 2017 Australasian Genomic Technologies Association (AGTA) in Hobart, Tasmania wrapped up last week and a common theme of the meeting was both applications and technical challenges of single-cell analysis.
In a new Cell Reports article, researchers utilized single-cell RNA-seq to help identify and characterize a subset of triple negative breast cancer (TNBC) tumors associated with sensitivity to EGFR-inhibition.
Profiling paired V(D)J transcripts from hundreds to millions of lymphocytes on a cell-by-cell basis, the Chromium™ Single Cell V(D)J system is a complete, scalable tool that has the potential to accelerate progress in immune therapy development.  In our recent 10x-pert Workshop, R&D scientists discussed the V(D)J Solution and performed a live demo of Loupe™ V(D)J Browser for data visualization.
Single-cell RNA-seq enables researchers to examine gene expression on a cell-by-cell basis providing insights into the function and regulation of complex biological systems.  In our recent 10x-pert Workshop, R&D scientists discussed single-cell RNA-seq data analysis tools, including Cell Ranger™ analysis pipelines, and performed a live demo of Loupe™ Cell Browser for data visualization.
Linked-Reads provide long-range information enabling structural variant detection and phasing of genome and exome sequencing data.  In our third 10x-pert Workshop session, our R&D 10x-perts provided an overview of the Loupe™ Genome Browser for structural variant detection and took closer look at visualizing simple structural variants and deriving complex events.
High quality high molecular weight (HMW) DNA is key to getting the most out of Linked-Read data. In a recent 10x-pert Workshop session, our R&D 10x-pert discussed general tips and best practices for isolating HMW DNA and took a closer look at two of our demonstrated protocols for HMW DNA isolation from fresh frozen tissues and DNA size selection.
We recently hosted our first live, online 10x-pert Workshop featuring single-cell sample prep topics.  Two of our R&D scientists gave a presentation detailing general do’s and don’ts followed by a closer look at two of our demonstrated protocols for preparing nuclei for single-cell RNA-seq and removing dead cells.
Have you ever wondered how to best prepare your samples or how much sequencing you need to achieve the highest quality Linked-Read data? We recently published a Bench Tips article with Biocompare, where we highlight important tips to make the most of our Linked-Read technology for long-range genomic analysis.
Two recent publications applied the power of Linked-Reads and the Chromium™ Genome Solution to genetically characterize gastric and triple-negative breast cancer. In both cases, the researchers discovered complex genomic rearrangements that they associated with the amplification of oncogenic driver genes.
New to 10x or need a refresher?  No problem.  Check out our new Chromium™ Single Cell 3’ training video series. Learn more about GemCode™ technology, single cell sample prep, data analysis and more.
In our June 22nd Nature Webcast, Dr. Aude Chapuis, who was recently awarded initial funding in a competition to develop projects that utilize single-cell RNA sequencing to improve research into immunotherapy-based cancer treatment, presented her T-cell immunotherapy research, highlighting the applications of scRNA-seq in developing new T-cell therapy approaches.
Five scientists from Fred Hutchinson Cancer Research Center were awarded the initial stage of funding in a competition sponsored by the Immunotherapy Integrated Research Center to develop projects that utilize single-cell RNA sequencing and 10x Genomics technology.
We are excited to announce the launch of our new Chromium™ Single Cell V(D)J Solution. It is the first high-throughput solution for profiling paired V(D)J transcripts from hundreds to millions of lymphocytes and expands our industry-leading single-cell product portfolio.
The InPSYght schizophrenia and bipolar disorder sequencing project seeks to produce Whole Genome Sequencing (WGS) data to identify variants associated with disease, build a reference panel of structural events and haplotypes to impute into large genotype data sets, and create and share data and methods with the community.
In a recent Nature Communications publication, researchers at the Fred Hutchinson Cancer Research Center and 10x use single cell analysis to profile Leukemia patients who had undergone bone marrow transplant.
The Chromium™ Genome and Exome Solutions use Linked-Reads to provide long range information from short read sequencers, giving you the power to detect complex SVs.
Linked-Reads provide the long range information missing from standard short read sequencing approaches, enabling reconstruction of long range haplotypes, detection of complex structural variants and the ability to perform de novo genome assembly.
  In recent years many technologies have been developed to look at individual cells at both the DNA and mRNA level to better understand what cell types are present in a tissue and how their malfunction can lead to disease.