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Single-cell RNA-seq enables researchers to examine gene expression on a cell-by-cell basis providing insights into the function and regulation of complex biological systems.  In our recent 10x-pert Workshop, R&D scientists discussed single-cell RNA-seq data analysis tools, including Cell Ranger™ analysis pipelines, and performed a live demo of Loupe™ Cell Browser for data visualization.
We recently hosted our first live, online 10x-pert Workshop featuring single-cell sample prep topics.  Two of our R&D scientists gave a presentation detailing general do’s and don’ts followed by a closer look at two of our demonstrated protocols for preparing nuclei for single-cell RNA-seq and removing dead cells.
Using single-cell sequencing and a battery of molecular tools, the authors achieved unprecedented insight into epithelial cell lineage relationships and the dynamics of differentiation in murine lacrimal gland development.