Welcome to the 10x Blog

From tips and tricks to 10x news, our blog is here to help you learn about all things 10x. Let us know what topics you would like to see in the Community Suggestions forum.

Our 3rd North American User Group Meeting of the year took place on Thursday, April 26th, in Chicago, and we were thrilled to see so many members of the 10x community there!
In a recent publication in Nature Communications, Porubsky, et al. combined strand-specific single-cell sequencing (Strand-seq) with long-read or Linked-Read sequencing to introduce an integrative method for phasing individual human genomes.
Linked-Reads provide long-range information enabling structural variant detection and phasing of genome and exome sequencing data.  In our third 10x-pert Workshop session, our R&D 10x-perts provided an overview of the Loupe™ Genome Browser for structural variant detection and took closer look at visualizing simple structural variants and deriving complex events.
High quality high molecular weight (HMW) DNA is key to getting the most out of Linked-Read data. In a recent 10x-pert Workshop session, our R&D 10x-pert discussed general tips and best practices for isolating HMW DNA and took a closer look at two of our demonstrated protocols for HMW DNA isolation from fresh frozen tissues and DNA size selection.
Have you ever wondered how to best prepare your samples or how much sequencing you need to achieve the highest quality Linked-Read data? We recently published a Bench Tips article with Biocompare, where we highlight important tips to make the most of our Linked-Read technology for long-range genomic analysis.
Although thousands of human genomes have been sequenced in the last decade, de novo assembly of individual genomes is not common due to the high cost and experimental burden.  In a recent Genome Research paper, Weisenfeld et al describe a straightforward, low cost method for creating true diploid de novo assemblies using 10x Genomics Linked-Reads and the Supernova™ assembler.
In a recent publication from the Broad Institute of MIT and Harvard, researchers identified >11,000 distinct structural variations among participants with autism spectrum disorder. These results provide new insights into the extent and diversity of structural variation present in the morbid human genome.
The InPSYght schizophrenia and bipolar disorder sequencing project seeks to produce Whole Genome Sequencing (WGS) data to identify variants associated with disease, build a reference panel of structural events and haplotypes to impute into large genotype data sets, and create and share data and methods with the community.
Check out our growing list of resources and learn how to make the most of the human genome with Linked-Reads.
Recently, we got to talk to Dr. Charlly Kao of CHOP about how Linked-Reads play a part in his current research, possible future applications, and why 10x technology is so exciting.
The Chromium™ Genome and Exome Solutions use Linked-Reads to provide long range information from short read sequencers, giving you the power to detect complex SVs.
Linked-Read data generated by the Chromium™ Genome Solution can be used for MHC analysis with Long Ranger™ Software.
Linked-Reads provide the long range information missing from standard short read sequencing approaches, enabling reconstruction of long range haplotypes, detection of complex structural variants and the ability to perform de novo genome assembly.
Haplotype analysis helps researchers to determine the order, structure and origin of variants across the genome and is important for advancing our understanding of human genetic variation.