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From tips and tricks to 10x news, our blog is here to help you learn about all things 10x. Let us know what topics you would like to see in the Community Suggestions forum.

The extensive collection of Illumina sequencing datasets, described by Bo Zhou and his co-authors (Alexander Urban Lab, Depts. of Psychiatry and Genetics) in a recent bioRxiv preprint, are derived from J. Craig Venter’s genome.
It’s User Group Meeting season; catch up on what happened in San Diego
In celebration of their upcoming 25th anniversary, The WellcomeTrust Sanger Institute will be collaborating with a number of leaders in the genomics field, including 10x Genomics, on their 25 Genomes Project, an exciting effort to comprehensively sequence 25 new genomes of species found in the UK.
In a recent publication in Nature Communications, Porubsky, et al. combined strand-specific single-cell sequencing (Strand-seq) with long-read or Linked-Read sequencing to introduce an integrative method for phasing individual human genomes.
In a recent Bioinformatics publication, researchers presented ARCS, a new application that uses 10x Linked-Reads to further organize draft genomes into highly contiguous assemblies.
The American Society of Human Genetics (ASHG) annual meeting continued in Orlando, FL and Thursday bought our 10x Software workshop "Intuitive Tools for Sequence Analysis: Crunching Genomic, Single-Cell, and Immune Repertoire Data Using 10x Chromium™ Software” and an exciting PerkinElmer workshop where they discussed "Linked-Reads: Enabling Robust Genome Analysis from Dried Blood Spots”.
Linked-Reads provide long-range information enabling structural variant detection and phasing of genome and exome sequencing data.  In our third 10x-pert Workshop session, our R&D 10x-perts provided an overview of the Loupe™ Genome Browser for structural variant detection and took closer look at visualizing simple structural variants and deriving complex events.
High quality high molecular weight (HMW) DNA is key to getting the most out of Linked-Read data. In a recent 10x-pert Workshop session, our R&D 10x-pert discussed general tips and best practices for isolating HMW DNA and took a closer look at two of our demonstrated protocols for HMW DNA isolation from fresh frozen tissues and DNA size selection.
Have you ever wondered how to best prepare your samples or how much sequencing you need to achieve the highest quality Linked-Read data? We recently published a Bench Tips article with Biocompare, where we highlight important tips to make the most of our Linked-Read technology for long-range genomic analysis.
Researchers at Stanford University have developed novel bioinformatics tools that leverage 10x Genomics’ Linked-Read sequencing to phase and resolve the complete structure of cancer genomic rearrangements. 
Two recent publications applied the power of Linked-Reads and the Chromium™ Genome Solution to genetically characterize gastric and triple-negative breast cancer. In both cases, the researchers discovered complex genomic rearrangements that they associated with the amplification of oncogenic driver genes.
There are many examples of how microbes influence the behavior of animals; however, studying the molecular basis for microbial behavior manipulation has suffered from a lack of tools. At our Bay Area User Group Meeting, Carolyn Elya spoke about establishing a D. melanogaster – E. muscae system and setting out to sequence and assemble the mind-controlling fungal pathogen.
There’s a growing number of data analysis tools being developed for single cell and Linked-Read applications, including gene expression, genome assembly, structural variant detection and more.  Check out the list we’ve compiled so far.
In a recent publication from the Broad Institute of MIT and Harvard, researchers identified >11,000 distinct structural variations among participants with autism spectrum disorder. These results provide new insights into the extent and diversity of structural variation present in the morbid human genome.
The InPSYght schizophrenia and bipolar disorder sequencing project seeks to produce Whole Genome Sequencing (WGS) data to identify variants associated with disease, build a reference panel of structural events and haplotypes to impute into large genotype data sets, and create and share data and methods with the community.
In a recent publication, researchers at the University of Hong Kong used 10x Linked-Reads in the prenatal identification of single gene disorders.
The Chromium™ Genome and Exome Solutions use Linked-Reads to provide long range information from short read sequencers, giving you the power to detect complex SVs.
Linked-Read data generated by the Chromium™ Genome Solution can be used for MHC analysis with Long Ranger™ Software.
We are excited to annouce the new Chromium Genome Kit v2, now available in an automation-friendly 96-reaction kit.  
Linked-Reads provide the long range information missing from standard short read sequencing approaches, enabling reconstruction of long range haplotypes, detection of complex structural variants and the ability to perform de novo genome assembly.