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From tips and tricks to 10x news, our blog is here to help you learn about all things 10x. Let us know what topics you would like to see in the Community Suggestions forum.

Our fourth North American UGM of 2018 was held on May 18th in New York, and we’re happy to report that it was packed, both with information and attendees! 
Our 4th North American User Group Meeting of the year took place on May 2nd in Houston, giving us a chance to check in with our Southwestern community of customers and researchers.
Our 3rd North American User Group Meeting of the year took place on Thursday, April 26th, in Chicago, and we were thrilled to see so many members of the 10x community there!
AACR 2018 is officially over! It was a great conference and the last day was no exception. 
AACR 2018 might be more than halfway over, but it didn’t show any signs of slowing down today. It was another jam-packed day full of highlights, starting with a Symposium on cell fate and treatment response
The first single-cell sequencing datasets from the Human Cell Atlas initiative are now available to the research community. Featuring data generated with 10x technology, the new datasets collect information from more than 500,000 cells.
From posters to talks to our Exhibitor Spotlight co-sponsored with Miltentyi Biotec Day 2 at AACR 2018 was all about single cell genomics!
Today was a big day for 10x at AACR 2018! Not only did I get to check out a bunch of great posters and talks featuring 10x technology, but I also got to attend the 10x Genomics Workshop, “Revealing the True Biology of Cancer."
Our second UGM of 2018 was held on March 14th in South San Francisco, where we were joined by more than 150 customers and researchers from throughout the Bay Area.
The Chromium Single Cell Immune Profiling Solution is a comprehensive approach to simultaneously examine the cellular context of the adaptive immune response and the immune repertoires of hundreds to millions of T and B cells on a cell-by-cell basis.  In this workshop 10x-perts Stephane Boutet and Sarah Taylor discussed how the Single Cell Immune Profiling Solution can be used to characterize the tumor microenvironment.
It’s User Group Meeting season; catch up on what happened in San Diego
Please join us on Tuesday, March 20th for the next 10x-pert workshop: Characterization of the tumor microenvironment with the Chromium Single Cell Immune Profiling Solution.
AGBT 2018 delivered some great science, talks, posters and time to catch up with old friends. Since our workshop was jam-packed with great information and exciting product announcements this year, we wanted to share some highlights.
Single-cell RNA-sequencing is a powerful tool for unraveling complex biological systems, but to take full advantage of this transformative technology, it is essential to properly prepare single-cell samples. In a recent Bench Tips article for Biocompare, we outlined some best practices for single-cell sample preparation.
We recently got the chance to talk to Vanessa Peterson, lead author on the Nature Biotechnology paper "Multiplexed quantification of proteins and transcripts in single cells", about the ideas behind REAP-seq, the possible applications of this new technique, and how 10x technology played a part in its development.
In a new publication in Nature Communications, researchers utilized single-cell RNA-seq to characterize the postnatal developmental shifts occurring in mouse mammary gland epithelial cells spanning pre-puberty, puberty, adulthood and pregnancy.
In a new publication in Blood Advances, researchers utilized the Chromium™ Single Cell 3’ Gene Expression Solution to explore immune surveillance in multiple myeloma and illustrate a novel pathway where immunomodulatory drugs may modulate the immune microenvironment.
Single-cell RNA-seq enables researchers to examine gene expression on a cell-by-cell basis providing insights into the function and regulation of complex biological systems.  In our recent 10x-pert Workshop, R&D scientists discussed single-nuclei RNA-seq, including when to choose single-nuclei RNA-seq, nuclei isolation protocols and best practices and data analysis considerations.
REAP-Seq is a novel assay that leverages barcoding technology to enable the simultaneous measurement of protein and RNA in single cells using the Chromium™ Single Cell 3’ Solution. The potential uses of the REAP-Seq assay are numerous and demonstrate the flexibility of the Chromium System for multiple single-cell applications.
We recently attended the EMBO | EMBL symposium “From Single- to Mulitomics: Appplications and Challenges in Data Integration” in Heidelberg, Germany. This conference provided a great platform to discuss applications, future ideas and challenges for integrating large-scale biological data across different biological technologies and disciplines.
The 2017 Australasian Genomic Technologies Association (AGTA) in Hobart, Tasmania wrapped up last week and a common theme of the meeting was both applications and technical challenges of single-cell analysis.
In a new publication in Nature, Broad Institute researchers utilized single-cell RNA-seq to identify and characterize novel small intestinal epithelial cell types and explore epithelial cell composition upon pathogen challenge and during homeostasis.
In a new Cell Reports article, researchers utilized single-cell RNA-seq to help identify and characterize a subset of triple negative breast cancer (TNBC) tumors associated with sensitivity to EGFR-inhibition.
The American Society of Human Genetics (ASHG) annual meeting continued in Orlando, FL and Thursday bought our 10x Software workshop "Intuitive Tools for Sequence Analysis: Crunching Genomic, Single-Cell, and Immune Repertoire Data Using 10x Chromium™ Software” and an exciting PerkinElmer workshop where they discussed "Linked-Reads: Enabling Robust Genome Analysis from Dried Blood Spots”.
Profiling paired V(D)J transcripts from hundreds to millions of lymphocytes on a cell-by-cell basis, the Chromium™ Single Cell V(D)J system is a complete, scalable tool that has the potential to accelerate progress in immune therapy development.  In our recent 10x-pert Workshop, R&D scientists discussed the V(D)J Solution and performed a live demo of Loupe™ V(D)J Browser for data visualization.
Single-cell RNA-seq enables researchers to examine gene expression on a cell-by-cell basis providing insights into the function and regulation of complex biological systems.  In our recent 10x-pert Workshop, R&D scientists discussed single-cell RNA-seq data analysis tools, including Cell Ranger™ analysis pipelines, and performed a live demo of Loupe™ Cell Browser for data visualization.
A recent PNAS publication from the laboratory of Dennis Lo applied the use of the 10x Genomics’ Chromium™ Single Cell 3’ Solution to comprehensively characterize the transcriptomic heterogeneity of the human placenta—an approach that served as confirmation for the non-invasive identification of cellular dysfunction during gestational complications such as preeclampsia.
We recently hosted our first live, online 10x-pert Workshop featuring single-cell sample prep topics.  Two of our R&D scientists gave a presentation detailing general do’s and don’ts followed by a closer look at two of our demonstrated protocols for preparing nuclei for single-cell RNA-seq and removing dead cells.
Transcriptome analysis has made the leap from bulk population-based studies to the single cell, and scientists are harnessing this new degree of resolution with remarkable ingenuity.  By analyzing the transcriptome of a single cell at a time, the heterogeneity of a sample is captured and resolved to the fundamental unit of living organisms—the cell.
In a new Cell Stem Cell publication, Yan et al, demonstrate the utility of single cell RNA-seq to study intestinal stem cell heterogeneity, lineage hierarchy and identify potential new stem cell markers.