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Single-cell RNA-seq enables researchers to examine gene expression on a cell-by-cell basis providing insights into the function and regulation of complex biological systems.  In our recent 10x-pert Workshop, R&D scientists discussed single-cell RNA-seq data analysis tools, including Cell Ranger™ analysis pipelines, and performed a live demo of Loupe™ Cell Browser for data visualization.
We recently hosted our first live, online 10x-pert Workshop featuring single-cell sample prep topics.  Two of our R&D scientists gave a presentation detailing general do’s and don’ts followed by a closer look at two of our demonstrated protocols for preparing nuclei for single-cell RNA-seq and removing dead cells.
In a new Cell Stem Cell publication, Yan et al, demonstrate the utility of single cell RNA-seq to study intestinal stem cell heterogeneity, lineage hierarchy and identify potential new stem cell markers.
New to 10x or need a refresher?  No problem.  Check out our new Chromium™ Single Cell 3’ training video series. Learn more about GemCode™ technology, single cell sample prep, data analysis and more.
A collaborative research study with Stanford University School of Medicine researchers was recently published in Nature. The article, “Non-equivalence of Wnt and R-spondin ligands during Lgr5+ intestinal stem-cell self-renewal,” describes how the 10x Genomics’ Single Cell 3’ Solution for single-cell RNA-seq (scRNA-seq) was utilized to help unravel the priming and self-renewal mechanisms of intestinal stem cells (ISCs).     
  In recent years many technologies have been developed to look at individual cells at both the DNA and mRNA level to better understand what cell types are present in a tissue and how their malfunction can lead to disease.