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From tips and tricks to 10x news, our blog is here to help you learn about all things 10x. Let us know what topics you would like to see in the Community Suggestions forum.

Today was a big day for 10x at AACR 2018! Not only did I get to check out a bunch of great posters and talks featuring 10x technology, but I also got to attend the 10x Genomics Workshop, “Revealing the True Biology of Cancer."
We are thrilled to announce the launch of the Chromium Single Cell CNV Solution. Combing high-throughput single cell profiling with genome-wide copy number variation (CNV) information, our new solution enables researchers to reveal genomic heterogeneity and understand clonal evolution.
Our second UGM of 2018 was held on March 14th in South San Francisco, where we were joined by more than 150 customers and researchers from throughout the Bay Area.
The Chromium Single Cell Immune Profiling Solution is a comprehensive approach to simultaneously examine the cellular context of the adaptive immune response and the immune repertoires of hundreds to millions of T and B cells on a cell-by-cell basis.  In this workshop 10x-perts Stephane Boutet and Sarah Taylor discussed how the Single Cell Immune Profiling Solution can be used to characterize the tumor microenvironment.
It’s User Group Meeting season; catch up on what happened in San Diego
Please join us on Tuesday, March 20th for the next 10x-pert workshop: Characterization of the tumor microenvironment with the Chromium Single Cell Immune Profiling Solution.
Elfin, Aurora and Qila leave their mark — Reference-Quality genome assemblies for the Northern Sea Otter and Beluga Whale inform conservation efforts and health studies
AGBT 2018 delivered some great science, talks, posters and time to catch up with old friends. Since our workshop was jam-packed with great information and exciting product announcements this year, we wanted to share some highlights.
Linked-Reads and the Supernova Assembler enable low-cost, everyday diploid de novo assemblies. In this workshop 10x-perts David Jaffe and Nikka Keivanfar discussed Supernova assembly performance across a range of species, including human, insect, plant and vertebrates, and highlighted improvements to our high molecular weight DNA sample preparation protocols, including a new protocol for single insects.
Single-cell RNA-sequencing is a powerful tool for unraveling complex biological systems, but to take full advantage of this transformative technology, it is essential to properly prepare single-cell samples. In a recent Bench Tips article for Biocompare, we outlined some best practices for single-cell sample preparation.
We were recently able to sit down and catch up with Hagen Tilgner about his team’s new manuscript that provides a more comprehensive understanding of the human transcriptome, “Microfluidic isoform sequencing shows widespread splicing coordination in the human transcriptome”, published in Genome Research.
We recently got the chance to talk to Vanessa Peterson, lead author on the Nature Biotechnology paper "Multiplexed quantification of proteins and transcripts in single cells", about the ideas behind REAP-seq, the possible applications of this new technique, and how 10x technology played a part in its development.
In celebration of their upcoming 25th anniversary, The WellcomeTrust Sanger Institute will be collaborating with a number of leaders in the genomics field, including 10x Genomics, on their 25 Genomes Project, an exciting effort to comprehensively sequence 25 new genomes of species found in the UK.
In a new publication in Nature Communications, researchers utilized single-cell RNA-seq to characterize the postnatal developmental shifts occurring in mouse mammary gland epithelial cells spanning pre-puberty, puberty, adulthood and pregnancy.
In a new publication in Blood Advances, researchers utilized the Chromium™ Single Cell 3’ Gene Expression Solution to explore immune surveillance in multiple myeloma and illustrate a novel pathway where immunomodulatory drugs may modulate the immune microenvironment.
In a recent Nature Communications publication, Hammond et al., report on sequencing, assembly and annotation of the draft North American bullfrog genome.
In a recent publication in Nature Communications, Porubsky, et al. combined strand-specific single-cell sequencing (Strand-seq) with long-read or Linked-Read sequencing to introduce an integrative method for phasing individual human genomes.
Single-cell RNA-seq enables researchers to examine gene expression on a cell-by-cell basis providing insights into the function and regulation of complex biological systems.  In our recent 10x-pert Workshop, R&D scientists discussed single-nuclei RNA-seq, including when to choose single-nuclei RNA-seq, nuclei isolation protocols and best practices and data analysis considerations.
REAP-Seq is a novel assay that leverages barcoding technology to enable the simultaneous measurement of protein and RNA in single cells using the Chromium™ Single Cell 3’ Solution. The potential uses of the REAP-Seq assay are numerous and demonstrate the flexibility of the Chromium System for multiple single-cell applications.
We recently attended the EMBO | EMBL symposium “From Single- to Mulitomics: Appplications and Challenges in Data Integration” in Heidelberg, Germany. This conference provided a great platform to discuss applications, future ideas and challenges for integrating large-scale biological data across different biological technologies and disciplines.
Researchers recently introduced a new software package for simulating Linked-Reads call LRSim. In a new paper by Luo et al., the authors demonstrate how the software can be used to optimize Linked-Read sequencing results.
The 2017 Australasian Genomic Technologies Association (AGTA) in Hobart, Tasmania wrapped up last week and a common theme of the meeting was both applications and technical challenges of single-cell analysis.
In a new publication in Nature, Broad Institute researchers utilized single-cell RNA-seq to identify and characterize novel small intestinal epithelial cell types and explore epithelial cell composition upon pathogen challenge and during homeostasis.
In a new Cell Reports article, researchers utilized single-cell RNA-seq to help identify and characterize a subset of triple negative breast cancer (TNBC) tumors associated with sensitivity to EGFR-inhibition.
In a recent Bioinformatics publication, researchers presented ARCS, a new application that uses 10x Linked-Reads to further organize draft genomes into highly contiguous assemblies.
The American Society of Human Genetics (ASHG) annual meeting continued in Orlando, FL and Thursday bought our 10x Software workshop "Intuitive Tools for Sequence Analysis: Crunching Genomic, Single-Cell, and Immune Repertoire Data Using 10x Chromium™ Software” and an exciting PerkinElmer workshop where they discussed "Linked-Reads: Enabling Robust Genome Analysis from Dried Blood Spots”.
The American Society of Human Genetics (ASHG) annual meeting started this week in Orlando, FL and there have been some exciting sessions, talks, posters and more.  We also had our first of two workshops featuring Hakon Hakonarson of CHOP and Madhuri Hedge of PerkinElmer.
The ASHG 2017 Presidential Symposium on Wednesday, October 18 featured speakers Bill Gates, Co-chair and Trustee of the Bill & Melinda Gates Foundation, and Francis Collins, Director of the U.S. National Institutes of Health, and was a lively conversation about global health and genomics.
The American Society of Human Genetics annual meeting took place from October 17 to 21 in Orlando, FL and we are excited to re-cap what 10x was up to including workshops, talks, posters and more.
Profiling paired V(D)J transcripts from hundreds to millions of lymphocytes on a cell-by-cell basis, the Chromium™ Single Cell V(D)J system is a complete, scalable tool that has the potential to accelerate progress in immune therapy development.  In our recent 10x-pert Workshop, R&D scientists discussed the V(D)J Solution and performed a live demo of Loupe™ V(D)J Browser for data visualization.