5', 3' RNA-seq and V(D)J libraries from single cells

Status: New
Posted By: jafar, on Nov 16, 2018 at 5:10 PM

Combining 5' and 3' chemistry should allow libray prep for both termini of transcripts from the same cell allowing better transcriptome characterisation. This should be easily achieved by using beads containing both1st strand synthesis primer and switch oligo with the same barcodes and some chemistry modification. This approach would allow V(D)J analysis as well proving 3 in 1 single cell transcrriptomic solution.

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