Chromium small genomes protocol

Status: New
Posted By: jafar, on Apr 13, 2017 at 6:22 PM

I wonder if current limitation on genome size for Chromium genome libraries can be overcome by using minimum recommended 600 pg input DNA for GEM generation and carrying on a genome size scaled portion of GEMs before or after amplification for the library prep. This approach would not disturb the expected number of fragments in GEMs or the read coverage/fragment. If it is feasible would 10x recommend any other modification to the protocols.


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