Feature Request: 8 base dual-index primers for 10X libraries

Status: New
Posted By: jayhesselberth, on Apr 27, 2018 at 8:44 AM

We are starting to run 10X libraries on a Nova-seq and there are some issues with the current indexing primers that make them unideal for the Nova-seq platform.


First, 10x libraries are single indexed. At scale, there are simply too many samples that would be put on a Nova-seq flow cells for single-indexes to be useful as they will likely collide with other libraries.


Second, indexes on the Nova-seq should not start with GG, because the Nova-seq is a two-color machine and G is the dark cycle; sometimes indexes that start GG can fail. 


I request that you move quickly to using dual 8 base indexes. There is minimal downside and it would future proof your libraries to be compatible with the Nova seq.


Let us know what you think about the new 10x Community in the Community Suggestions forum.