Feature Request: 8 base dual-index primers for 10X libraries

Status: New
Posted By: jayhesselberth, on Apr 27, 2018 at 8:44 AM

We are starting to run 10X libraries on a Nova-seq and there are some issues with the current indexing primers that make them unideal for the Nova-seq platform.

 

First, 10x libraries are single indexed. At scale, there are simply too many samples that would be put on a Nova-seq flow cells for single-indexes to be useful as they will likely collide with other libraries.

 

Second, indexes on the Nova-seq should not start with GG, because the Nova-seq is a two-color machine and G is the dark cycle; sometimes indexes that start GG can fail. 

 

I request that you move quickly to using dual 8 base indexes. There is minimal downside and it would future proof your libraries to be compatible with the Nova seq.

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