Genome Protocol mixing of smaller genomes

Status: New
Posted By: Tom-UU-Sweden, on Sep 22, 2016 at 11:54 PM

This is just an idea but if I understand correctly the Genome kit is optimised to the human genome (~3.3Gb) and there is a problem with running smaller non-human genomes below 400Mb. Could you combine two or more unrelated genomes in one sample to come up to the aboutthe same 3.3Gb of the human. Then I guess you would have a bioinformatics headache to separate them after, but technically would it work?

 

Tom

Comments
Posted By: clementfkent, on Feb 1, 2017 at 10:18 AM

I don't know the answer, but I can speculate a bit.

 

I suspect the unrelated genomes would have to be sufficiently divergent from each other that post-sequencing assembly can be done unambiguously. I hope someone at 10x can comment on this, because Tom's idea seems seductively easy...

Posted By: Emmasmith, on Nov 19, 2018 at 11:13 AM

umm! thats want i want know aslo hope is there anyone who can help me out too .

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