Ribosome footprinting (Ribo-mRNA-Seq) for targeting only actively translated mRNA at single cell res

Status: New
Posted By: abenham, on Aug 5, 2017 at 12:28 PM

Could your labelling application be modified to first select active ribosome complexes and only tag or label the RNA or mRNAs undergoing active translation? It would also nice if you could  label the RNA that is not undergoing active translation from the same sample. You could see how variation in  gene expression at the translational level by which proteins are actually being made can distinguish the various sub cell types that make up a particular tissue or maybe even distinguish early developmental patterns....


Let us know what you think about the new 10x Community in the Community Suggestions forum.