TruSeq-Nextera hybrid library prep

Status: New
Posted By: jafar, on Sep 30, 2016 at 6:58 PM

Current Chromium library prep steps (specially single cell) are labour intensive. I wonder if you have considered a hybrid library prep with Nextera where amplified cDNA or barcoded genome fragments are tagmented  and  PCR amplified with one of Nextera indexed primers and a TruSeq adapter primer. Considering different structure of genome and single cell fragments opposing Nextera and TruSeq adapter would be used for each library.

Comments
Posted By: abaffyp, on Feb 13, 2018 at 4:44 AM

If you have money for it - they haven't option for kit with only PCR amplification of WTA library - you can use it. In theory - Bio-Rad develop together with Illumina instrument and kit which use tagmentation (which is better than fragmentation + ligation), and it's works. But, Bio-Rad's instrument have a lot of limitation in comparsion with 10x, so it could be better to have "open" version of kits for 10x instrument.

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