Current Chromium library prep steps (specially single cell) are labour intensive. I wonder if you have considered a hybrid library prep with Nextera where amplified cDNA or barcoded genome fragments are tagmented and PCR amplified with one of Nextera indexed primers and a TruSeq adapter primer. Considering different structure of genome and single cell fragments opposing Nextera and TruSeq adapter would be used for each library.
You must be a registered user to add a comment. If you've already registered, sign in. Otherwise, register and sign in.