TruSeq-Nextera hybrid library prep

Status: New
Posted By: jafar, on Sep 30, 2016 at 6:58 PM

Current Chromium library prep steps (specially single cell) are labour intensive. I wonder if you have considered a hybrid library prep with Nextera where amplified cDNA or barcoded genome fragments are tagmented  and  PCR amplified with one of Nextera indexed primers and a TruSeq adapter primer. Considering different structure of genome and single cell fragments opposing Nextera and TruSeq adapter would be used for each library.

Posted By: abaffyp, on Feb 13, 2018 at 4:44 AM

If you have money for it - they haven't option for kit with only PCR amplification of WTA library - you can use it. In theory - Bio-Rad develop together with Illumina instrument and kit which use tagmentation (which is better than fragmentation + ligation), and it's works. But, Bio-Rad's instrument have a lot of limitation in comparsion with 10x, so it could be better to have "open" version of kits for 10x instrument.


Let us know what you think about the new 10x Community in the Community Suggestions forum.