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Re: List of valid cellular barcodes

Posted By: juan-du, on Apr 10, 2019 at 3:04 PM

Dear @hisplan,

 

Thank you for your question.

 

The reason we see 6.8 million barcodes in the whitelist is that in Gene Expression v3 chemistry beads, the 10x barcodes for mRNA capture and 10x barcodes for feature barcode capture by design have a mismatch of 2 bp.
For example, the 10x barcode AAACCCATCCGATGTA for GEX library has AAACCCAAGCGATGTA in the feature barcode library. The whitelist contains both the GEX and feature library version of 10x barcodes and hence the file has twice the number of barcodes in the whitelist.

 

I hope this answers your question.

 

Best Regards,

Juan

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Re: List of valid cellular barcodes

Posted By: hisplan, on Apr 12, 2019 at 7:17 AM

Hi Juan,

 

Thanks for your quick response and detailed explanation.

 

Is there a way to distringuish between the 10x barcodes for mRNA capture and 10x barcodes for feature barcode capture? (i.e. create a separate file for each)

 

Thanks!