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During cDNA amplification, why is the elongation time only 1 minute?

Posted By: EricBrenner, on May 29, 2019 at 3:11 PM

One minute might be long enough for the majority of mature mRNAs, but it wouldn't be long enough to amplify a lot of pre-mRNAs, right? This would be especially problematic when working with nuclei instead of whole cells as you'd probably fail to amplify a large proportion of your template molecules. Right?

 

Referring to page 32 of the v3 protocol: https://assets.ctfassets.net/an68im79xiti/51xGuiJhVKOeIIceW88gsQ/1db2c9b5c9283d183ff4599fb489a720/CG...

 

Cheers,

 

-Eric