Demostrated Methanol fixation protocol for single applications

Posted By: Luciano2018, on Jul 21, 2018 at 11:11 PM
Dear all,

I have a doubt about this one. Organic solvents such as methanol, acetone and ethanol preserve cells through a process of dehydration and precipitation of proteins. These fixatives have been shown to be very effective at preserving nucleic acid content in cells, but at the cost of cell membrane structure, the loss of cytoplasmic organelles and nuclear content. Organic solvents like these then permeabilise cells by removing lipids from the cell membrane (and have the added advantage that they also simultaneously fix cells, thereby only requiring a one-step process). How is that methanol fixed cells maintain the membrane integrity that is needed for single cell applications?