Does normalization by total UMI take care of sequencing depth?

Posted By: yzz, on Aug 20, 2018 at 7:31 AM

I know the cellranger aggr function can be used to adjust sequencing depths of different samples. But runing aggr each time a new sample coming in doesn't seem to be realistic. Any normalization method do you recommend that can be applied to sample individually? So I can add a new sample to existing data set without runing normalization for all samples again. Thanks!