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8 Replies

Re: ‘Frankenstein’ protocol for nuclei isolation from fresh and frozen tissue-version 3 kit

Posted By: eseifie, on Oct 16, 2019 at 3:25 AM

Thank you

Re: ‘Frankenstein’ protocol for nuclei isolation from fresh and frozen tissue-version 3 kit

Posted By: Luciano2018, on Oct 26, 2019 at 12:56 PM
Hi Shauna, could this (How to set up 3’ v3) be updated on Frankenstein? Thank you!

Re: ‘Frankenstein’ protocol for nuclei isolation from fresh and frozen tissue-version 3 kit

Posted By: eseifie, on Oct 28, 2019 at 4:02 AM

The Nuclei EZ lysis buffer that was used in the Frankenstein protocol is not sold seperately anymore by Millipore Sigma but instead it is a kit. Has anyone tried the Kit yet? 

Re: ‘Frankenstein’ protocol for nuclei isolation from fresh and frozen tissue-version 3 kit

Posted By: Luciano2018, on Oct 28, 2019 at 1:06 PM
Hi, it’s is the same thing. They call it ‘kit’ but it’s simply the lysis buffer and nuclei storage buffer.

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