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Single nuclei RNAseq from frozen tissues

Posted By: Luciano2018, on Sep 20, 2018 at 1:55 PM
Hi all,

Here it is. Good luck, keep me posted and feel free to get in touch if you need help. Luciano

https://community.10xgenomics.com/t5/Customer-Developed-Protocols/ct-p/customer-protocols
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Re: single nuclei RNAseq from frozen tissues

Posted By: ronychanoch, on Oct 14, 2018 at 1:42 AM

Hi Luciano,

 

I am also interested in doing 10x on single nuclei of tumors (not brain).
I would be super greatful if you can share any progress/protocol that you have worked on.


Thanks!

Rony

Re: single nuclei RNAseq from frozen tissues

Posted By: Luciano2018, on Oct 14, 2018 at 1:46 AM
Hey Rony,

The protocol I use can be found here:

https://community.10xgenomics.com/t5/Customer-Developed-Protocols/ct-p/customer-protocols

Happy for help of you need to chat further.

Luciano

Re: single nuclei RNAseq from frozen tissues

Posted By: ronychanoch, on Oct 14, 2018 at 2:17 AM

Thanks so much! I will check it out Smiley Happy

Re: single nuclei RNAseq from frozen tissues

Posted By: standfirm30, on Dec 4, 2018 at 5:08 AM

Hi Luciano,

 

Thanks so much for your great work on nuclei isolation and share the protocol.

 

Best,

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